Table 3.
Capsid proteins VP1 and VP3 alanine mutants tested in cell culture and in vitro
| Virus variant (genetic locus) | Amino acid location | 50% Tissue culture infectious dose (TCID)/mla | Replicationb | Translationc |
|---|---|---|---|---|
| Wild-type | N/A | 106 | Yes | Yes |
| VP1 | R202A | 0 | Yes | Yes |
| VP1 | C203A | 0 | Yes | Yes |
| VP3 | T17A | 106 | Yes | Yes |
| VP3 | Y19A | 0 | Yes | Yes |
| VP3 | W21A | 0 | Yes | Yes |
| VP3 | T44A | 0 | Yes | Yes |
| VP3 | R55A | 0 | Yes | Yes |
| VP3 | R68A | 0 | Yes | Yes |
aValues are the mean TCID50 values calculated from two independent experiments
bReplication efficiency of each mutant was tested by staining with anti-dsRNA antibody and by quantitative real time PCR
cTranslation efficiency of each mutant was tested by phosphorimaging of in vitro translation of mutants labelled with 35S-methionine