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. 2017 May 12;7:1848. doi: 10.1038/s41598-017-02085-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of LL-37 on the surface expression of ICAM-1 and CD18 on eosinophils and BEAS-2B cells in the co-culture. Human eosinophils (3 × 10⁵) and BEAS-2B cells (1 × 10⁵) were cultured either together or separately with or without 2 or 10 μg/ml LL-37 for 20 h. (A) representative dot plot and histograms illustrating differential flow cytometric analysis of surface expression of ICAM-1 and CD18 on eosinophils or BEAS-2B cells in the co-culture of triplicate independent experiments showing essentially similar results. (B,C) quantitative results of surface ICAM-1 (B) or CD18 (C) expression on eosinophils and BEAS-2B cells are presented as MFI. EOS, eosinophils; CoEOS, eosinophils in co-culture; CoBEAS-2B, BEAS-2B cells in co-culture. Results are shown as arithmetic mean plus SD of triplicate independent experiments. *P < 0.05, **P < 0.01 when compared between denoted groups and eosinophil/fibroblast control groups. ^## P < 0.01 when compared between denoted groups and co-culture control groups.