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. 2017 May 12;7:1848. doi: 10.1038/s41598-017-02085-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of transwell inserts on cytokine/chemokine expressions in co-culture of eosinophils and BEAS-2B cells upon the stimulation of LL-37. Human eosinophils (3 × 10⁵) and BEAS-2B cells (1 × 10⁵) were cultured together with or without 10 µg/ml LL-37 in the presence or absence of transwell inserts for 20 h. Release of IL-6 (A), CXCL8 (B) and CCL4 (C) in culture supernatant was determined. Results are shown as arithmetic mean plus SD of triplicate independent experiments. **P < 0.01 when compared between the denoted group and the corresponding control groups without treatment.