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. 2017 May 12;7:1848. doi: 10.1038/s41598-017-02085-5

Figure 6.

Figure 6

Inhibitors for p38-MAPK, NF-κB and ERK-MAPK pathways abolished LL-37-induced activation of human eosinophils co-cultured with BEAS-2B cells. Human eosinophils (3 × 105) co-cultured with BEAS-2B cells (1 × 105) were pretreated with 7.5 μM SB203580 (p38-MAPK inhibitor), 1 μM BAY11-7082 (NF-κB inhibitor), 10 μM U0126 (ERK inhibitor) for 1 h, followed by incubation with 10 µg/ml LL-37 for further 20 h. ICAM-1 expression on eosinophils (A) and BEAS-2B cells (B) as well as CD18 expression on eosinophils (C) was analyzed and expressed as previously described. Release of IL-6 (D), CXCL8 (E) and CCL4 (F) in the culture supernatant was determined. BAY, BAY11-7082; SB, SB203580; U, U0126. Results are shown as arithmetic mean plus SD of triplicate independent experiments. *P < 0.05, **P < 0.01 when compared between the denoted groups and the corresponding control groups with LL-37 treatments. DMSO was served as solvent control.