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. 2017 May 10;7:1687. doi: 10.1038/s41598-017-01870-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DMBA induced DNA damage and apoptosis in mature oocytes from COCs. (a) Representative images of γH2A.X immunostaining. Scale bar, 500 μm. (b) Average number of DSB foci per mature oocyte. (c) The lysates of 200 oocytes collected at 44 h from control and DMBA treated COCs were subjected to western blot analysis and bands of γH2A.X and β-tubulin were cropped out of blot to display (full-length blot as shown in Supplementary Fig. S3). (d) Relative quantification of γH2A.X protein level. (e) Representative images of Annexin-V staining (green) in mature oocytes. Scale bar, 200 μm. (f) Percentages of apoptosis positive oocytes. (g) The relative mRNA level of Bax, Bcl2 and Caspase3 in mature oocytes. *P < 0.05; **P < 0.01 and ***P < 0.001.