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. 2017 May 10;7:1652. doi: 10.1038/s41598-017-01520-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identifying tuft cells for volumetric electron microscopy. (A) Overview of correlative method to identify a specific cell by fluorescence, then performing targeted scanning electron microscopy to uncover the ultrastructure of the desired cell in the third dimension. (B) A tuft cell in the colonic epithelium of ChAT-GFP transgenic mice is identified by fluorescence (B-left) and ATUM SEM (B-center), then volume rendered from serial images using data visualization software (B-right). (C) Volumetric EM analysis of tuft cells revealed cytospinules and a gut-to-endoplasmic reticulum passage. Bars in B-left and middle = 10 µm, bar in B-right = 1 µm.