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. 2017 May 10;7:1652. doi: 10.1038/s41598-017-01520-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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A molecular passage to the endoplasmic reticulum. (A) An overview of a passage in the tuft cell connecting its endoplasmic reticulum to the gut lumen. (B) A continuous tubular passage starting at the base of the tuft cell microvilli (left) becomes evident after volume rendering of ATUM images (right). (B) Glycocalceal bodies (arrows) were observed within microvilli as well as within the tubular passage. (D) Volume rendering of ATUM images reveals a passage in tuft cells. It is formed by tubules that start at the base of the microvilli and extend to the endoplasmic reticulum. The tubules appear to merge with the endoplasmic reticulum but this cannot be discerned at the current resolution (4 nm/pixel). See also Video 2. Bars = 1 µm.