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. 2017 May 12;7:1808. doi: 10.1038/s41598-017-01872-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Top half covered growth (TCG) test results revealed that light perception in roots did not promote DWF4 expression in the root apex. (a) Root elongation. Red arrowheads indicate the junctions between the hypocotyl and root, while yellow arrowheads indicate the root apex positions. Hypocotyl (b) and root (c) lengths according to the TCG test. The grey background indicates which part was darkened [n = 32]. Means denoted by the same letter are not significantly different (P < 0.05) according to the Tukey–Kramer method. DWF4-GUS expression around the shoot apical meristem (d–h) and in the root apex (i–m). Light conditions with no film (d,i) or with a clear film (e,j). Shoots in darkness covered with a black film and roots under light (f,k). Although the root apex was exposed to light, the GUS signal in the root meristem was weaker than that of plants that were incubated entirely under light. Dark conditions with no film (g,l) or with a clear film (h,m). Bars represent 10 mm (a) and 50 µm (d–m). Error bars represent standard deviations.