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. 2017 May 11;7:1787. doi: 10.1038/s41598-017-01973-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PSC display constitutive activation of STAT3 and MAPK signaling and secrete pro-inflammatory factors. (a) Summary of all PSC and PSC cell cultures used. (b) Cells were grown in DMEM and lysed once they reached 70–80% confluence for western blot analysis. pSTAT3, STAT3, pERK, and ERK were analyzed by immunoblot. β-actin served as a loading control. (c) Supernatants from 3 murine PSC cell lines and the human control pancreas-derived fibroblast line (HFP) (detailed in Table 1) were collected from 70% confluent cells grown in DMEM. A panel of cytokines and chemokines was analyzed in these supernatants by Luminex assay. Values are listed as an average of two replicates in pg/mL with the standard deviation. ND indicates analytes were assayed, but the levels were detected beneath the lowest standard curve reference value. (d) Selected results from the Luminex assay, presented as a heat map.