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. 2017 May 12;7:1878. doi: 10.1038/s41598-017-01731-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fluorescence quantification of CFSE molecules on labeled EVs. (A) Background reference noise and EV fluorescence intensity transformed into a MESF histogram, see Supplementary Figure 6 for additional analysis. (B) Fluorescence detection range of the 95% of total EVs and (C) average MESF + SEM of EVs stained with CFSE, from four independent experiments. MESF, Molecules of Equivalent Soluble Fluorochrome.