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. 2017 May 12;7:1810. doi: 10.1038/s41598-017-01813-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SDS-PAGE (10–15% acrylamide) of the four purification stages. L: cell lysate, F: flow-through of the first affinity chromatography step, P: insoluble cell material, M: molecular weight marker; Weights of the used standard protein are indicated left of the gel., AC: eluate of the first affinity chromatography step, l: latent AbPPO4 (eluate of the second affinity chromatography step), a: activated AbPPO4 (eluate of size exclusion chromatography after activation with proteinase K).