Table 1.
Details of endongenous candidate reference genes.
| Name* | Length (bp) | Accession number | Primer | qPCR efficiency mean (SE) | Correlation coefficient mean R2 (SE) |
|---|---|---|---|---|---|
| ssa-miR-17-5p | 23 | MIMAT0032414 | F: caaagtgcttacagtgcaggtag | 98.98 (0.006) | 0.99 (0.002) |
| ssa-miR-23a-3p | 21 | MIMAT0032549 | F: atcacattgccagggatttcc | 98.97 (0.012) | 1.00 (0.002) |
| ssa-miR-30e-5p | 22 | MIMAT0032611 | F: tgtaaacatcctacactcagct | 98.95 (0.002) | 0.99 (0.005) |
| ssa-miR-99-5p | 23 | MIMAT0032718 | F: aacccgtagatccgatcttgtg | 98.98 (0.003) | 0.99 (0.002) |
| ssa-miR-183-5p | 22 | MIMAT0032429 | F: tatggcactggtagaattcact | Not included | Not included |
| ssa-miR-214-5p | 22 | MIMAT0032514 | F: tgcctgtctacacttgctgtgc | 99.00 (0.022) | 0.99 (0.002) |
| ssa-let-7a-5p | 22 | MIMAT0032688 | F: tgaggtagtaggttgtatagtt | 98.99 (0.019) | 0.99 (0.001) |
| 18S rRNA | 21 | AJ427629.1 | F: cgatcagataccgtcgtagtc | 99.00 (0.014) | 0.99 (0.002) |
| 18 | R: cagccttgcgaccatact | ||||
| U6 | 20 | BT047885 | F: ttcgcgatggaagaacgcta | 98.97 (0.007) | 1.00 (0.001) |
| 20 | R: aacctgctgcaagactgtgt |
*MicroRNA names in accord with50.