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. 2017 May 10;7:1653. doi: 10.1038/s41598-017-01966-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The relationship between the location of PTC and NMD. (a) The distance of PTC from start codons are shown for sig-neg-AFD mutations (red) and the other mutations (gray). (b) Scatterplot shows the distance of PTC to downstream exon junction. The x-axis is represented as a cumulative sum. The point is marked on every 10 nts. (c) The sig-neg-AFDs ratio are plotted against the mRNA half-life. The minutes on the x-axis are represented as a cumulative scale.