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. 2017 May 11;7:1749. doi: 10.1038/s41598-017-01866-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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WISP1 gene expression is decreased during adipocyte differentiation. (a) The expression of WISP1 mRNA was measured in subcutaneous (SAT) and epididymal adipose tissues (VAT) harvested from 12 weeks old C57BL6 mice (n = 4–5 per group). (b) Preadipocytes isolated from VAT and SAT were induced to differentiate. Total RNA was isolated and the expression of WISP1 and ADIPOQ mRNA were measured by real-time PCR. The values indicate the changes for the indicated samples compared to VAT on Day 0. (c) 3T3-F442A cells were induced to differentiate and the gene expression of WISP1, and ADIPOQ was measured by real-time PCR. The values indicate the changes for the indicated samples compared to day 0. (d) Secreted and intracellular protein levels of WISP1 were analyzed during adipocyte differentiation by ELISA. (e) Expression of secreted and intracellular protein levels of WISP1 and adiponectin were measured by Western Blotting. Adiponectin serves as a positive control for adipocyte differentiation while actin was used as a loading control. All values are representative of data from 3 independent experiments each performed in duplicate. Results are presented as means ± SEM *p < 0.05; **p < 0.01; ***p < 0.005.