Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 May 12;7:1844. doi: 10.1038/s41598-017-02090-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

TEM analysis of PaCS autophagy and bleb formation in IL4-DC after IL-4 withdrawal. TEM of cells incubated for 5 days with GM-CSF plus IL-4 and then for 3 (A–D) or 5 (E–G) days in RPMI–FBS without cytokines. (A) A clear area, not delimited by membrane, was seen in the cytoplasm of a DC. This was identified as an empty PaCS, due to its residual barrel-like particles and 20S immunogold reactivity, visible at higher magnification in the inset. (B–D) Autophagic double or multilayered membranes penetrate and wrap/envelope cytoplasmic areas containing FK1-positive PaCS. See structural details in the enlargement (C1) and p62 immunoreactivity of an autophagosomal vesicle with fused multilayered membranes in (D). (E–G) A collection of empty autophagic vesicles and no PaCS are seen in (E), while in (F) a single-membrane vesicle shows heterogeneous, lysosome-type dense bodies and sparse, residual immunogold reactivity for the autophagic marker LC3. (G) FK1-reactive PaCS were still recognized in the cytoplasm as well as in the cell blebs, although most barrel-like particles were dissolved. Nuclear and cytoplasmic densification, with loss of details and organelles, were indicative of ongoing apoptosis. See enlargement in (G1). n, nucleus.