Table 1. Multiplex PCR primers.

Gene	Mutation sites	Primer sequences	Size (bp)	PCR tube
GJB2	c.35delG, c.167delT, c.176_191del16, c.235delC, c.299-300delAT	HL1F:5’- AGAGCAAACCGCCCAGAGTAGAA -3’ HL1R: 5’-GAAGATGACCCGGAAGAAGATGCT -3’	420	Tube I
SLC26A4	c.281C>T	HL4F: 5’- GGCTCCCCAAATACCGAGT -3’ HL4R: 5’- TGGTAGCTGGGGAGAAGTG -3’	150	Tube I
	c.589G>A	HL5F: 5’- CAGCTAGAGTCCTGATTGCCA -3’ HL5R: 5’- GCCTTAATAAGTGGGGTCTTGC -3’	250	Tube I
	IVS7-2A>G	HL6F: 5’- CAGCATTATTTGGTTGACA -3’ HL6R: 5’- CCCTTGGGATGGATTTA -3’	304	Tube I
	c.2162C>T, c.2168A>G	HL10F: 5’- AATGCGGGTTCTTTGACGACA -3’ HL10R: 5’- AAATGGAACCTTGACCCTCTTGA -3’	115	Tube I
	c.1174A>T, c.1226G>A, c.1229C>T	HL7F: 5’- GGACCACCACGCAGAGTAG -3’ HL7R: 5’- TGCCATTCCTCGACTTGTTCTC -3’	287	Tube II
	IVS15+5G>A	HL8F: 5’- CAGTCCTATTTTCTATGGCAATGTC -3’ HL8R: 5’- TGCCCTACACAAAGGGAAGAGG -3’	177	Tube II
	c.1975G>C, c.2027T>A	HL9F: 5’- TGCTTACCAAGGAACAGTGTGT -3’ HL9R: 5’- GCCCATGTATTTGCCCTGTTG -3	334	Tube II
GJB3	c.538C>T, c.547G>A	HL2F: 5’- GCCCCCTGCCCCAACATCGTG -3’ HL2R: 5’- GTGGCAGCGGCAGGTGGAAGC -3’	200	Tube II
MTRNR1	m.1555A>G, m.1494C>T	HL3F: 5’- TTAAGGGTCGAAGGTGGATTTAG -3’ HL3R: 5’- TGGTTTGGCTAAGGTTGTCTGGTA -3’	367	Tube II