Fig 1. AlamarBlue metabolic activity, QRT-PCR gene expression, and ALP activity in BM-MSCs isolated from young (≤50 years)(a-e) and old (≥70 years)(f-j) donors seeded on decellularized bone scaffolds from either young (≤50 years) or old (≥70 years) bone donors.

Relative metabolic activity (a, f) was normalised to non-seeded bone controls and day 0 readings whilst relative gene expression of osteogenic markers RUNX2 (b, g), OPN (c, h) and OC (d, i) were normalised to the reference gene MRPL19 and day 0 controls (2^-ΔΔCT). Relative ALP enzymatic activity (e, j) was normalised to non-seeded bone controls, DNA content and day 0 readings. Data represent mean ± SE. * indicates significant difference with respect to 0d control (p≤0.05). # indicates significant difference between young and old samples at the same time-point (p≤0.05).