Table 1.
Correlation of viral RNA and histopathological lesions in brains of BoAstV CH13/NeuroS1 positive feedlot cattle with non-suppurative encephalitis
| Case IDa | Brain structure | BoAstV CH13/NeuroS1 in-situ hybridization gradeb | Histopathology | |||
|---|---|---|---|---|---|---|
|
| ||||||
| Grade of inflammation | Meningitis | Gliosis | ||||
|
| ||||||
| Percent of blood vessels with peri-vascular cuffs (%)c | Number of cell layers in the peri-vascular cuffsd | |||||
| 31 | Medulla oblongata | 1 | 1 | +++ | N | Y |
| Midbraine,f | 3 | 1 | +++ | N | Y | |
| Cerebellume | 0 | 1 | +++ | N | Y | |
| Cerebrum | 3 | 0 | — | N | N | |
| Thalamus | 3 | 1 | + | N | Y | |
| 37 | Medulla oblongata | 0 | 1 | +++ | N | N |
| Cerebellum | 2 | 0 | — | N | Y | |
| Cerebrum | 3 | 0 | — | N | N | |
| 35 | Medulla oblongata | 0 | 1 | + | N | Y |
| Midbraine | 2 | 1 | + | N | Y | |
| Cerebellum | 0 | 0 | — | Y | Y | |
| Cerebrume | 1 | 1 | + | Y | Y | |
| Hippocampus | 0 | 1 | + | N | N | |
| Thalamuse | 2 | 2 | + | N | Y | |
| 23 | Medulla oblongata | 0 | 1 | +++ | N | Y |
| Midbraine | 2 | 1 | ++ | N | Y | |
| Cerebellum | 0 | 0 | — | N | Y | |
| Cerebrum | 1 | 0 | — | N | N | |
| Hippocampus | 3 | 0 | — | N | N | |
| Thalamus | 1 | 0 | — | N | N | |
Case identification from Sanchez et al (2).
ISH grading (0–3): 0 — astrovirus negative; 1 — mild astrovirus infection (1 to 10 cells stained positive); 2 — moderate astrovirus infection (11 to 50 cells); 3 — severe astrovirus infection (> 50 cells).
Percentage of affected blood vessels per section: 0 — none; 1 — < 25%; 2% — 25% to 50%; 3 — > 50%.
Number of cell layers in the perivascular cuffs: + — scant number of cells not forming a complete concentric ring around the vessel to 1 complete cell layer; ++ — 2 to 3 complete cell layers; +++ — more than 3 complete cell layers.
Glial nodules present
Neuronophagia observed.
Y — present; N — absent.