Extended Data Figure 3. Histology of the testes at day 28 after infection with ZIKV H/PF/2013.

Seven week-old WT C57BL/6 mice were treated with PBS or anti-Ifnar1 at day -1 prior to subcutaneous inoculation in the footpad with 10³ FFU of ZIKV H/PF/2013 or 10⁶ FFU of DENV-2. Testes were collected at day 14 (a) or 28 (b) after infection and analyzed for viral RNA by qRT-PCR. Results are pooled from two independent biological experiments and each symbol represents data from an individual mouse. Bars indicate mean values. c. Histological analysis of PFA-fixed testis (left panels) and epididymis (right panels) tissues collected from uninfected or ZIKV-infected animals at day 28 at 20× (left) and 40× (right) magnification. Arrows indicate loss of germ cells and vacuoles in the testis (red), involution of epididymal lumens (yellow) with a mass of residual sperm (blue) and thickened epithelium (green). The images are representative of several independent experiments. Scale bars are indicated in the bottom right corner of the panels. Scale bars = 200 μm.