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. Author manuscript; available in PMC: 2017 May 15.
Published in final edited form as: Mol Microbiol. 2005 Jul;57(1):238–249. doi: 10.1111/j.1365-2958.2005.04673.x

Fig. 1. ClpX modulates FtsZts assembly in vivo.

Fig. 1

A. A clpX null mutation restores Z-ring formation to ftsZts cells at 45°C. PL642 (ftsZts), BW182 (ftsZts spx::neo), BW189 (ftsZts spx::neo clpX::spc) and BW206 (ftsZts spx::neo clpP::cat) cells were cultured in LB at 30°C to mid-exponential phase (OD600 ~0.400) and then back-diluted into LB at 30°C or 45°C and grown for ~2.5 h. FtsZts (FtsZ–GFP) was visualized in fixed cells using fluorescence microscopy. Arrows indicate examples of medial Z rings. Scale bar = 2.5 μm.