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. Author manuscript; available in PMC: 2017 May 15.
Published in final edited form as: Mol Microbiol. 2005 Jul;57(1):238–249. doi: 10.1111/j.1365-2958.2005.04673.x

Fig. 2. ClpX overexpression blocks Z-ring assembly.

Fig. 2

A. ClpX overexpression increases cell length. Cells encoding both an IPTG-inducible clpX allele and an ftsZts allele (BW243) were grown in LB at 37°C with 10 μM IPTG to maintain basal levels of ClpX expression. At mid-exponential phase (OD600 ~0.400) cultures were back-diluted into LB containing either 10 μM IPTG (grey bars) or 1 mM IPTG (black bars) and grown for ~2.5 h prior. FtsZts (FtsZ– GFP) was visualized in live cells using fluorescence microscopy. Arrows indicate Z rings in cells grown in LB with 10 μM IPTG. Scale bar = 2.5 μm.