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. Author manuscript; available in PMC: 2017 May 15.
Published in final edited form as: Mol Microbiol. 2005 Jul;57(1):238–249. doi: 10.1111/j.1365-2958.2005.04673.x

Fig. 5.

Fig. 5

FtsZ interacts with, but is not degraded by, ClpXP.

A. ClpXP is able to degrade Spx but not FtsZ in vitro. Proteolysis reactions were performed at 37°C in the presence of 5 mM ATP and sampled at 0 and 60 min.

B. The presence of 10-fold mass excess of FtsZ relative to Spx inhibits the degradation of Spx by ClpXP. Proteolysis reactions were performed at 30°C and sampled at 0, 5, 10, 15, 30, 45 and 60 min. Samples in (A) and (B) were analysed by SDS-PAGE and stained with coomassie Brilliant Blue and representative gels are shown.