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. 2017 May 6;6:e18931. doi: 10.7554/eLife.18931

Figure 2. SHN-1 binding to EGL-19’s carboxy-terminus promotes the expression or function of L-type calcium channels.

(A) Predicted c-terminal sequences of mutant EGL-19 proteins are shown. egl-19(nu496) is a 22 bp deletion and egl-19(nu495) is a 5 bp deletion, both resulting in frame shifts that delete the carboxy-terminal PDZ ligand of EGL-19 (-VTTLCOOH). Residues in blue represent the PDZ ligand. Residues in red represent those introduced by the frame shift mutations. (B–E) Voltage-activated Ca+2 currents were recorded from adult body wall muscles of the indicated genotypes at holding potentials of −60 to +40 mV. Representative traces (B), mean current density at 0 mV (C, E), and mean current density as a function of holding potential (D) are shown. The egl-19(nu496) and shn-1(tm488) single mutants had similar decreases in Ca+2 current-density, and additive defects were not observed in the double mutant. The number of animals analyzed is indicated for each genotype. Values that differ significantly from wild type controls are indicated (***p<0.001). Error bars indicate SEM. Mean, standard errors, sample sizes, and p values for this figure are shown in Supplementary file 1.

DOI: http://dx.doi.org/10.7554/eLife.18931.004

Figure 2.

Figure 2—figure supplement 1. Supplemental data related to Figure 2.

Figure 2—figure supplement 1.

Deleting the EGL-19 c-terminal PDZ ligand (in nu496 mutants) had no effect on the kinetics of current deactivation (A) nor on the voltage dependence of current activation (B). Summary data are plotted for recordings shown in Figure 2. (C–F) The shn-1(ok1241) mutation, which deletes the PDZ and part of the proline rich domains (Figure 1—figure supplement 1D), decreases calcium current density (C–D) but has no effect on the voltage-dependence of current activation (E) nor on the kinetics of current deactivation (F). Values that differ significantly from wild type controls are indicated (**p<0.01, *p<0.05). Error bars indicate SEM. Mean, standard errors, sample sizes, and p values for this figure are shown in Supplementary file 1.
DOI: http://dx.doi.org/10.7554/eLife.18931.005