Figure 6. DSF in combination with copper inhibits SUM149-derived spheroids grown in a high-throughput assay format.
SUM149 cells plated at 400 cells/well in a 384-well ultra low attachment plate treated with indicated compounds and includes a 10-pt dose response, 1:2 dilutions of Staurosporine (0.002 μM – 1 μM); CuSO4 (0.3 μM – 160 μM), DSF alone (0.01 μM – 5 μM); and combination of DSF (0.01 μM – 5 μM) + 10 μM CuSO4 (DSF+Cu) and single concentration of GANT 61 (10 μM). Representative images A., B. of Hoechst and YOYO-1 channels merged for a combined assessment of spheroid area, integrity and growth inhibition are shown along with C. graphical presentation of the quantitative multiparametric analysis.