Table 1. Multiparametric cell health measurements of the multicellular tumor spheroids derived from IBC cells grown in low-attachment plate analyzed in the high content assay.
| Dye | Emission Color (Wavelength) | Measurement | Cellular Determinant | Spheroid/Individual Cell Parameter |
|---|---|---|---|---|
| Hoechst 33342 | Blue (386 nm) | Mean_Object Size Mean_Object Shape LWR Mean_Object Shape P2A Mean_Object Variable Intensity Ch1 |
Size Nuclear Aspect Ratio Roundness (Perfect circle = 1) Texture |
Morphology |
| Hoechst 33342 | Blue (386 nm) | Object count/Field | Spheroid density per valid field | Proliferation |
| YOYO-1 | Green (485 nm) | Mean_Target Average Intensity, Ch 2 (YOYO-1 pixel intensity normalized to spheroid size) |
Plasma membrane integrity | Viability |
| Mitotracker FM Red | Red (549 nm) | Mean_Target Average Intensity, Ch 3 Mean_Target Total Intensity, Ch 3 (Mitotracker pixel intensity normalized to spheroid size) |
Average brightness of functional mitochondria Total mass of functional mitochondria | Mitochondrial Function/Membrane Potential |