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. 2016 Apr 18;8(16):25885–25896. doi: 10.18632/oncotarget.8834

Figure 1. BCL-9 expression in different developing stages of colorectal cancer tissue.

Figure 1

Representative immunohistochemical stained imaging of BCL-9 expression in different developing stages of colorectal cancer tissue containing 284 cases were shown. Positive cells were stained brown. (A) BCL-9 expression in normal mucosa. (B) low intensity expression in colorectal cancer cells scored as “−”. (C) moderate intensity expression in colorectal cancer cells scored as “+”. (D). high intensity expression in colorectal cancer cells scored as “++”. (E) Positive and negative control of BCL-9 expression showed the specificity of staining and were validated by Western-blot assays. Scale bar: 40 μm for low magnitude images (10×); 10 μm for high magnitude images (40×).