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. 2016 Apr 18;8(16):25885–25896. doi: 10.18632/oncotarget.8834

Figure 2. Hypoxia induces BCL-9 expression levels in human colorectal cancer cell lines.

Figure 2

Human colorectal cancer cell lines SW480 and HCT116 cells were cultured under the hypoxic condition for the indicated time periods. (A) The mRNA expression levels of BCL-9 in these cells were determined by Taqman real-time PCR and normalized with actin. (B) The mRNA expression levels of VEGF in these cells were determined as a positive control. (C) The BCL-9 protein levels were determined by Western-blot assays. Data are presented as mean ± SD (n = 3). *p < 0.01, Student's t-test.