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. 2016 Apr 18;8(16):25885–25896. doi: 10.18632/oncotarget.8834

Figure 5. HIF-1α activates the induction of BCL-9 expression by hypoxia.

Figure 5

Knockdown of endogenous HIF-1α but not HIF-2α largely abolishes the induction of BCL-9 expression by hypoxia in HCT116 cells. Cells with knockdown of endogenous HIF-1α, HIF-2α by siRNA oligos or transfected with control siRNA were treated with hypoxia for 36 h. Two different siRNA oligos against HIF-1α and HIF-2α, respectively, were used, and similar results were obtained. (A) The mRNA expression levels of BCL-9 (left panel) and VEGF (right panel) were determined by Taqman real-time PCR and normalized with actin. (B) The BCL-9 protein levels were determined by Western-blot assays. (C) The knockdown of HIF-2α (left panel) and HIF-1α (right panel) in cells was conformed at the mRNA level by Taqman real-time PCR and normalized with actin. Data are presented as mean ± SD (n = 3). *p < 0.01 (Student's t-test).