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. 2016 Apr 18;8(16):25885–25896. doi: 10.18632/oncotarget.8834

Figure 6. HIF-1α expression is associated with BCL-9 expression in human colorectal cancer specimens.

Figure 6

HIF-1α and BCL-9 protein levels were examined on specimens of 244 cases of human colorectal cancer by the double immunofluorescence (IF) co-localization. (A) Representative IF staining results for HIF-1α (upper panels) and BCL-9 (lower panels) are shown. Positive HIF-1α or BCL-9 staining: > 25% cells stained with HIF-1α or BCL-9, respectively. (B) Positive and negative control of BCL-9 expression showed the specificity of staining and were validated by Western-blot assays. Scale bar: bar = 10 μm. (C) HIF-1α expression is associated with BCL-9 expression in human colorectal cancer (p < 0.000, Pearson chi-square test).