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. 2016 Nov 4;8(16):25897–25914. doi: 10.18632/oncotarget.13071

Figure 3. No regrowth/relapse in Rb1- or compound K-treated SKOV-3 and HEYA8 spheroids.

Figure 3

A. Expression of CSC markers Bmi-1, Nanog, and Oct4 after Rb1 or CK treatment. β-actin serves as an internal control. The signal intensity was determined by densitometry. B. SKOV-3 and HEYA8 spheroids untreated or treated with Rb1 or compound K (CK) were plated as secondary spheroids. Representative views of secondary spheroids imaged until day 6 (right). The number of tumor spheres generated was photographed and counted (left). Bar, 50 μm. Experiments were repeated three times, and data are shown as mean ± SD. *, P < 0.05 vs control.