Table 2. Analysis of the genomic potential off-targets of Bcr-Abl sgRNA.
Gene | Annotation | Target | Forward | Reverse |
---|---|---|---|---|
Rcor1 | NM_198023 | GGAAATCACAAGCCCTTCAGCAG | ATGGCAGCTCTCCCAATCAACA | TACAAGCACCAGGTACAATGA |
Epg5 | NM_001195633 | AGTGTTGAATAACCCTTCAGGGG | CAGTAGCAGTGTTGCCCTCCA | CACACCTTCTCTTAGTCTATGG |
Gimap7 | NM_146167 | AGAACTCAAAGGCCCTGCAGGAG | CTAAGCAACATGTGGACCACT | GGAGCTGAAGGGAGTTAGGG |
Zfp457 | NM_001003666 | AGTGTACAAAAGCTCTTCAGTGG | GCGACTAAGATGGTTTCAATAG | GCACAGCACCTCAAGGTCAG |
Alg10b | NM_001033441 | AGAGCCCACAACCCCTTCAGCGG | AGTGCTCCTAAGGGGCTTAGT | GAAGTAAGCGGCATTGCTTATAG |