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. 2017 Feb 16;8(16):26129–26141. doi: 10.18632/oncotarget.15387

Figure 2. ZNF521 depletion impairs cell proliferation, induces cell cycle arrest but not apoptosis in MLL-rearranged cell lines.

Figure 2

(A) MTT cell viability assay in the MLL leukemic cells THP-1, NOMO-1, OCI-AML4 and ML2 transduced with ZNF521 shRNAs (ZNF004 or ZNF710) or non-targeting scramble control (shScram). GFP+ cells were sorted 4 days after transduction and placed in appropriate medium. Graphs show percentage of GFP+ cells measured at day 4, day 7 and day 10, normalized to the percentage of shScram cells. Data are represented as mean ± SD of at least three independent experiments. *P < 0.05, **P < 0.001, ***P < 0.0001, t-test. (B) Colony formation of GFP+ cells transduced with ZNF521 shRNAs or shScram. Error bars represent mean ± S.D. of three independent experiments. **P < 0.001, ***P < 0.0001, t-test. (C) Cell cycle distribution at day 7 of ZNF521 knockdown cells and control shScram of gated GFP+ cells. Data are represented as mean ± SD of three independent experiments. **P < 0.001, ***P < 0.0001, t-test. (D) Percentage of apoptotic cells (Annexin V+/DAPI- and Annexin V+/DAPI+) measured after 4 and 7 days post-transduction of gated GFP+ cell population. Data are represented as mean ± SD of three independent experiments.