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. 2017 Feb 16;8(16):26142–26153. doi: 10.18632/oncotarget.15393

Figure 5. Andrographolide induced cell cycle arrest and apoptosis in colon cancer cells is ROS dependent.

Figure 5

(A) T84 and COLO 205 cells were pretreated with or without NAC followed by Andro IC50 (45 μM) for 24 h and 48 h. Cell lysates were analyzed by immunoblot and quantified by densitometry for expression of Cyclin B1 and cyclin A. Expression is normalized against GAPDH expression. (B) T84 and COLO 205 cells were pretreated with or without NAC followed by Andro IC50 for 24 h and 48 h. Cells were then lysed and incubated with caspase 3 substrate and activity was determined by colorimetric assay at OD405nm. (**P < 0.01, ***P < 0.001).