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. 2017 Feb 19;8(16):26434–26447. doi: 10.18632/oncotarget.15507

Figure 2. RAB10 knockdown induced cell cycle arrest and apoptosis and reduced colony formation in SMMC-7721 cells.

Figure 2

Control-shRNA, cells infected with negative control shRNA lentivirus; shRAB10, cells infected with RAB10 shRNA lentivirus. (A) RAB10 mRNA expression levels were detected by qRT-PCR in human normal hepatic cell line (LO2) and four HCC cell lines (SMMC-7721, Huh-7, Hep 3B and Hep G2). (B) RAB10 protein levels in SMMC-7721 cells and RAB10 mRNA levels in SMMC-7721 cells were knocked down efficiently by shRNA. (C) Knockdown of RAB10 expression induced G0/G1 phase arrest. (D) Knockdown of RAB10 increased apoptosis. (E) Knockdown of RAB10 reduced colony formation. Data were shown as means ± SD (n = 3), *P < 0. 05, **P < 0.01, ***P < 0.001.