Table 3. The combination of the kinase inhibitor AC220 with tunicamycin synergistically promotes apoptosis of FLT3ITD-expressing but not wild-type FLT3-expressing 32D cells.

Compound treatment		32D cells FLT3ITD	32D cells Wild-type FLT3
AC220 (nM)	Tunicamycin (μg/ml)	Annexin V assay (CI)
0.5	0.5	0.822	0.991
1	0.5	0.796	1.033
2	0.5	0.323	1.038

32D cells either expressing FLT3ITD or wild-type FLT3 cells were treated simultaneously with the indicated concentrations of AC220 and tunicamycin for 24 h. Subsequently apoptosis was determined using the Annexin V method. Combination indices were determined according to the method of Chou-Talalay [31]. Combinations with synergism (CI < 1) are highlighted grey.