Figure 4. EGFR inhibitors attenuated the LPS-induced acute lung injury in rats.

SD rats were treated intragastrically with compound AG1478 or 451 at a dosage of 20 mg·kg⁻¹ b.wt or vehicle (0.5% CMC-Na) for one week, then challenged with or without 5 mg·kg⁻¹ LPS. Rats were euthanized with ketamine after 24 h of LPS induction. (A) Histopathological changes in lung tissues determined by H&E staining. (B) The lung injury score was determined. (C) Lung wet/dry ratio was detected. (D–F) BAL fluid was collected, and protein content (D), total cells (E) and neutrophils number (F) were measured. (G) MPO activity in lung tissue homogenates was measured. Data are mean ± SEM of 4-6 separate animals. *p< 0.05, **p < 0.01 and ***p < 0.001 vs. only-LPS stimulated group.