Figure 6. AG1478 and 451 reduced LPS-induced inflammation in rats.

SD rats were treated intragastrically with compound AG1478 or 451 at a dosage of 20 mg·kg⁻¹ b.wt or vehicle (0.5% CMC-Na) for one week, then challenged with or without 5 mg·kg⁻¹ LPS. Rats were euthanized with ketamine after 24 h of LPS induction. (A and B) The inflammatory cytokines TNF-α in BALF (A) and serum (B) were determined by ELISA. (C–I) Total mRNA was extracted from the lung tissue using TRIzol and the mRNA levels of TNF-α (C), IL-6 (D), IL-1β (E), IL-8 (F), ICAM-1 (G), VCAM-1 (H) and MCP-1 (I) were detected by real-time RT-qPCR analysis. (J) Macrophages infiltration in lung tissue was detected by CD68 immunohistochemical assay. Data are mean ± SEM of 4-6 separate animals. *p < 0.05, **p < 0.01 and ***p < 0.001 vs. only-LPS stimulated group.