Table 1. Primer sequences for real-time quantitative PCR.
Gene | Species | FW | RW |
---|---|---|---|
TNF-α | human | CCCAGGGACCTCTCTCTAATC | ATGGGCTACAGGCTTGTCACT |
IL-6 | human | GCACTGGCAGAAAACAACCT | TCAAACTCCAAAAGACCAGTGA |
IL-1β | human | ACGCTCCGGGACTCACAGCA | TGAGGCCCAAGGCCACAGGT |
IL-8 | human | CTCTTGGCAGCCTTCCTGATT | ACTCTCAATCACTCTCAGTTCT |
ICAM-1 | human | GAACCAGAGCCAGGAGACAC | TCCCTTTTTGGGCCTGTTGT |
VCAM-1 | human | GGCGCCTATACCATCCGAAA | TATGACCCCTTCATGTTGGC |
MCP-1 | human | TTCCCCTAGCTTTCCCCAGA | TCCCAGGGGTAGAACTGTGG |
COX-2 | human | TTCTCCTTGAAAGGACTTATGGGTAA | AGAACTTGCATTGATGGTGACTGTTT |
β-Actin | human | CCTGGCACCCAGCACAAT | GCCGATCCACACGGAGTACT |
TNF-α | rat | TACTCCCAGGTTCTCTTCAAGG | GGAGGCTGACTTTCTCCTGGTA |
IL-6 | rat | GAGTTGTGCAATGGCAATTC | ACTCCAGAAGACCAGAGCAG |
IL-1β | rat | GGGCCTCAAGGGGAAGAATC | ATGTCCCGACCATTGCTGTT |
IL-8 | rat | GAAGATAGATTGCACCGATG | CATAGCCTCTCACACACATTTC |
ICAM-1 | rat | GCCTGGGGTTGGAGACTAAC | CTGTCTTCCCCAATGTCGCT |
VCAM-1 | rat | AGGTTGGGGATTCCGTTGTT | ACACATTAGGGACCGTGCAG |
MCP-1 | rat | GTCACCAAGCTCAAGAGAGAGA | GAGTGGATGCATTAGCTTCAGA |
β-Actin | rat | AAGTCCCTCACCCTCCCAAAAG | AAGCAATGCTGTCACCTTCCC |