Figure 5. CDC42 and CACNA2D2 expression in SW620 tumor xenografts.

(A) Analysis of total CDC42 protein levels in xenograft tumors generated by our SW620 cellular model of CDC42 knock down (CDC42-i1 and CDC42-i3), CDC42 overexpression (CDC42-wt1) and parental SW620 as well as in the cell lines prior injection. Western blot images of immunoblots stained with CDC42 and normalized using GAPDH as loading control. Three representative tumors (T1-T3) of each group are shown. The original images of these Western blots are shown in Supplementary Figure 4B. (B) Probe specificity for human CACNA2D2 gene was tested using a murine cell line (MBT2) as negative control and two different human cell lines (SW620 and CDC42-i1 clone). (C) CACNA2D2 gene expression was determined in the different tumor xenografts. CACNA2D2 expression levels in cell lines before injection are shown as reference. CACNA2D2 levels were normalized with the human-specific housekeeping gene PGK1. The results are shown as Log₁₀ of relative quantity (RQ) of CACNA2D2 in each sample using control SW620 cell line as reference