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. 2017 Mar 1;8(16):26802–26818. doi: 10.18632/oncotarget.15818

Figure 3. DNA methyltransferases are not involved in DNA demethylation process mediated by SET accumulation.

Figure 3

. (A) Immunofluorescence assay was performed in HEK293, HEK293/SET, HN12, HN12siSET, HN13, and HN13siSET cells to determine the levels of DNMT1, the enzyme responsible for DNA methylation maintenance. Cells from five fields were measured using ImageJ software. Graphics represent the percentage of DNMT1-positive cells. Red arrows indicate SET accumulation; green arrows indicate SET downregulation. (B) DNA methyltransferases enzymatic activity assay using nuclear protein extract from cells; this assay was perfomed using EpiSeeker DNMT Activity Quantification Kit (Abcam), according to the manufacturer's protocol. Graphic represents experiments performed in triplicate. *(p < 0.05),**(p < 0.01) and ***(p < 0.001).