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. 2017 Mar 6;8(16):27240–27251. doi: 10.18632/oncotarget.15916

Figure 6. LPA induced expression of IL-17 by LPAR signaling in splenocytes from NOD mice.

Figure 6

(A) Splenocytes were treated with the indicated doses of LPA for 24 h, and mRNA expression of IL-17 was analyzed by qRT-PCR. (B) Splenocytes were treated with or without 10 μM LPA and 10 μM Ki16425 for 24 h, and mRNA expression of IL-17 was analyzed by qRT-PCR. (C) Under conditions as for (B), splenocytes were re-stimulated with 1 μg/ml PMA and 2 μg/ml ionomycin for 6 h, and the supernatants were analyzed for IL-17 by ELISA. Means ± SD (n=3/group, *p<0.05, **p<0.01, ***p<0.001).