Figure 5. Effects of HMGB1 on pulmonary infiltration in mice.

A. Experimental scheme shows twenty-four mice divided into four groups that received different treatments. Note: i.p. = intraperitoneal injection. B. Hematoxylin & Eosin staining of lung tissue sections. After 6-days of treatments, the mice from the four groups were sacrificed, the lungs were removed and tissue sections were prepared as described in the methods. After staining with hematoxylin & eosin, the specimens were examined microscopically under high power field (400X magnification). C. Immunohistochemistry of ICAM-1 in lung tissue sections of mice from the four treatments was performed as described in the methods. The specimens were examined microscopically under 400X magnification. The lung vascular endothelial cells were marked by CD31 antibody. The arrows indicate positive immunoreactivity of ICAM-1 and/or CD31.