Table 1. EBER RNA expression according to the anatomic localization of HNSCC and presence of EBV DNA (PCR), multiple- or single types of HPV (HR- or LR type) or HSV-1 by PCR. Percentages given in parentheses present the positivity calculated from the total number of samples. The results of LMP-1 immunostaining are given as the number of cases with positive staining present in over 50% of the cancer cells.
| Site/n | EBER-ISH + carcinoma | EBER-ISH + lymphocytes | EBV PCR + | LMP-1 IHC + | HPV multiple | HPV single | HPV negative | HSV-1 PCR + |
|---|---|---|---|---|---|---|---|---|
| Lip n=5 | ||||||||
| EBV +++ | - | - | - | - | - | - | - | - |
| EBV + | - | - | - | - | - | - | - | - |
| EBV - | 5 (100%) | 2 (40%) | 4 (80%) | 1 (20%) | 3 (60%) | 2 (40%) | - | - |
| Oral n=24 | ||||||||
| EBV +++ | - | - | - | - | - | - | - | - |
| EBV + | 2 (8%) | 2 (8%) | 2 (8%) | 1 (4%) | - | 1 (4%) | 1 (4%) | - |
| EBV - | 22 (92%) | 8 (33%) | 14 (58%) | 4 (17%) | 7 (29%) | 8 (33%) | 8 (33%) | 1 (4%) |
| Oropharynx n=19 | ||||||||
| EBV +++ | - | - | - | - | - | - | - | - |
| EBV + | 2 (11%) | 2 (11%) | 1 (5%) | 1 (5%) | - | - | 2 (11%) | - |
| EBV - | 17 (89%) | 5 (26%) | 11 (58%) | 3 (15%) | 2 (11%) | 7 (37%) | 8 (42%) | 2 (11%) |
| Hypopharynx n=9 | ||||||||
| EBV +++ | - | - | - | - | - | - | - | - |
| EBV + | 1 (11%) | 1 (11%) | - | - | - | 1 (11%) | - | - |
| EBV - | 8 (89%) | 3 (33%) | 7 (78%) | 1 (11%) | 3 (33%) | 1 (11%) | 4 (44%) | - |
| Nasopharynx n=8 | ||||||||
| EBV +++ | 6 (75%) | 2 (25%) | 6 (75%) | 6 (75%) | 1 (13%) | 1 (13%) | 4 (50%) | - |
| EBV + | 1 (13%) | 1 (13%) | 1 (13%) | - | - | 1 (13%) | - | - |
| EBV - | 1 (13%) | 1 (13%) | - | 1 (13%) | - | - | 1 (13%) | - |
| Larynx n=8 | ||||||||
| EBV +++ | 1 (13%) | 1 (13%) | 1 (13%) | 1 (13%) | 1 (13%) | - | 1 (13%) | - |
| EBV + | 1 (13%) | 1 (13%) | N/A | - | - | 1 (13%) | - | - |
| EBV - | 6 (76%) | - | 4 (50%) | - | - | 2 (25%) | 3 (38%) | - |
N/A = Data not available