Figure 8. Bapta-AM potentiates dexamethasone-induced inhibition of ERK1/2 signaling by chelating Ca2+ signaling in primary blasts from ALL patients.

(A, B) Primary blasts were exposed to 100 nM dexamethasone (Dex) with or without preincubated (20 minutes) with Bapta-AM (5 μM) in Ca²⁺ buffer and the changes in [Ca²⁺]i were monitored as Figure 5. (C) ALL blasts were preincubated (20 min) with Bapta-AM (5 μM) in Ca²⁺ buffer and then exposed to 100 nM Dex for 5 min. Cell lysates were subjected to western blotting analysis with the indicated antibodies, along with quantification (D). Data represent the mean ± SEM (n=3).