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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: J Tissue Eng Regen Med. 2017 May 31;12(1):59–69. doi: 10.1002/term.2363

Figure 1. Formation of scaffold-free tissue sheet with parallel cell and matrix organization.

Figure 1

Light micrographs of (a) top view and (b) cross sectional view of the PDMS substrate show grooves approximately 10 μm wide, 10 μm apart, and 5 μm deep. (c) Phase contrast image shows CSSC cultured on the grooved substrate. (e) For better visualization, CSSC were labeled with DiI (red) and cultured on grooved substrate. (e) Two-photon micrograph of 10-day cultures of CSSC on grooved substrates in keratocyte differentiation medium (KDM) shows deposition of parallel organized collagenous matrix (green). Nuclei (blue) were stained by SYTOX-green (blue). (f) After 10 days of culture a robust tissue sheet is formed that can be separated from the substrate using forceps. Scale bars: (a) and (b) = 50 μm, (c)–(e) = 100 μm