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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: J Tissue Eng Regen Med. 2017 May 31;12(1):59–69. doi: 10.1002/term.2363

Figure 5. Persistence of human cells and matrix in mouse cornea stroma after implantation.

Figure 5

Five weeks after implantation of CSSC generated tissue sheets, mouse corneas were harvested and analyzed as described under Methods. (a) Confocal image of whole mount of cornea shows DiO labelled human cells (green). All cells were stained using nuclear dye DAPI (blue). (b) Higher magnification of boxed region in (a) shows region where human cells are located and in (c) a cross sectional projection of dotted line in (b) shows that the human cells are localized to the central stroma. (d) Immunostaining human keratocan (red) in cryosections from corneas 5 weeks after tissue sheet implantation. (d′) Micrograph of green channel in same region as (d) shows DiO labelled human cells (green), (d″) micrograph of DAPI nuclear staining of all cells in same region as (d), and (d″′) a merged image of (d)–(d″) show keratocan staining localization with respect to the human cells. (e) Immunostaining human keratocan (red) in a section of control mouse cornea lacking scaffold-free tissue sheets. (e′) Micrograph of same region as (e) showing lack of DiO labeled green cells (green), (e″) micrograph in same region as (e) of DAPI nuclear staining of all cells (blue), and (e″′) merged image of (e)–(e″). Scale bars: (a) = 500 μm, (b) = 100 μm, (d)–(e) = 20 μm