Figure 6.

STAT1 is required for IFNγ‐mediated inhibition of neurosphere growth.

STAT1‐KO NSPCs were treated with IFNγ (1–1000 U/ml) or with heat‐inactivated IFNγ (ΔH IFNγ; 1000 U/ml) as a negative control for DIV 3 and 5 post‐IFNγ treatment A: Representative images of neurospheres imaged 5 days post‐IFNγ treatment at 2x magnification for different concentrations of IFNγ (Scale bar = 250 μm). B: Quantitation of neurosphere diameter at 3 and 5 days post‐IFNγ treatment. The longest diameter of each neurosphere was measured using Image J software. Data was collected from neurospheres in five fields/condition from three biological replicates and graphed as a percentage of untreated controls. Statistical analysis was applied by one‐way ANOVA (***p < 0.001, ****p < 0.0001). C: Histogram plots of neurosphere area for different concentrations IFNγ on DIV 5 as measured by pixel² for each neurosphere. Frequency distribution of the number of neurospheres corresponding to the indicated neurosphere area in pixel² was plotted for each condition. Note that the range of the x‐axis is greater than in Figure 1C.