Figure 8.

IFNγ activates STAT3, but does not inhibit cyclin E expression or pRb phosphorylation at S795, in the absence of STAT1.

STAT1‐KO NSPCs were treated with IFNγ (100U/ml) and collected on DIV 2, 3, and 5 for western blot analysis. Representative blots for phosphorylated and total STAT3 A: cyclins D1, D2, D3, E and cdk2, B: and for total pRb and associated pRb phosphorylation sites, and C: are shown. Signal intensity for each band was normalized to GAPDH as a loading control. Quantitation of signal intensity is shown as the average from 3 independent biological replicates with SEM. Statistical analysis was applied using repeated measures one‐way ANOVA with Bonferroni multiple comparisons post‐hoc analysis (*p < 0.5, ****p < 0.0001).