Table 1.

Characterization of Antibodies Used in Flow Cytometry and Western Blot Analyses.

Antigen	Molecular weight (kDa)	Immunogen	Source (RRID)	Concentration	Characterization
anti‐nestin PE conjugated antibody	200	E. coliderived recombinant rat Nestin: Met544Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys)	R and D Systems Cat# IC2736P (AB_2151131)	1:50	Mutnal et al. Murine cytomegalovirus infection of neural stem cells alters neurogenesis in the developing brain. Nestin was detected in murine NSPCs using flow cytometry
anti‐doublecortin antibody	40	Amino acids 162‐441 at the C‐terminus of doublecortin of human origin	Santa Cruz Biotechnology Cat# sc‐28939, (AB_2088488)	1:50	Maire et al. Pten Loss in Olig2 Expressing Neural Progenitor Cells and Oligodendrocytes Leads to Interneuron Dysplasia and Leukodystrophy. Doublecortin was detected in paraffin frozen sections of mouse brain using immunofluorescence
anti‐phospho STAT1	91,84	Phosphorylated Human Stat1 (pY701) Peptide	BD Biosciences Cat# 612133 Clone 14/P‐STAT1 (AB_399504)	1:1000	Lin et al. Distinct Roles of Type I and Type III Interferons in Intestinal Immunity to Homologous and Heterologous Rotavirus Infections. phospho STAT1 was detected in mouse lung, kidney and liver using western blot
anti‐STAT1‐total	91,84	STAT1 aa 1‐94	BD Biosciences Cat# 610120 (AB_397526)	1:1000	Grant et al. Zika Virus Targets Human STAT2 to Inhibit Type I Interferon Signaling. STAT1 was detected in 293T cells using western blot
anti‐STAT3‐total. Two isoforms, alpha(85kDa) beta (79 kDa)	86,79	Stat3 aa. 1‐175	BD Biosciences Cat# 610190 (AB_397589)	1:1000	Ye et al. Wnt/β‐catenin and LIF‐Stat3 signaling pathways converge on Sp5 to promote mouse embryonic stem cell self‐renewal. STAT3 was detected in mouse embryonic stem cells derived from preimplantation embryos using western blot
anti‐phospho STAT3 (Two isoforms, alpha and beta	86,79	Synthetic phosphopeptide corresponding to residues surrounding Tyr705 of mouse Stat3	Cell Signaling Cat# 9131 (AB_331587)	1:1000	Fukuda et al. Potentiation of Astrogliogenesis by STAT3‐Mediated Activation of Bone Morphogenetic Protein‐Smad Signaling in Neural Stem Cells. phospho STAT3 was detected in mouse neuroepitherlial cells and differentiating cells derived from E.14 embryos using western blot
anti‐phospho STAT3 (Two isoforms, alpha and beta	86,79	Synthetic phosphopeptide corresponding to residues surrounding Tyr705 of mouse Stat3	Cell Signaling Cat# 9131 (AB_331587)	1:1000	Fukuda et al. Potentiation of Astrogliogenesis by STAT3‐Mediated Activation of Bone Morphogenetic Protein‐Smad Signaling in Neural Stem Cells. phospho STAT3 was detected in mouse neuroepitherlial cells and differentiating cells derived from E.14 embryos using western blot
anti‐phospho STAT1 (S727)	91,84	Synthetic phosphopeptide corresponding to residues surrounding Ser727 of human Stat1	Cell Signaling Cat# 9177 (AB_2197983)	1:1000	Rosowski et al. Toxoplasma gondii Inhibits Gamma Interferon (IFN‐γ)‐ and IFN‐β‐Induced Host Cell STAT1 Transcriptional Activity by Increasing the Association of STAT1 with DNA. Phospho STAT1 (S727) was detected in 293 FT and HEK cells using western blot.
anti‐cyclin D1	36	Synthetic peptide corresponding to the carboxy‐terminus of human cyclin D1.	Cell Signaling Cat# 2978 (AB_2259616)	1:1000	Kosmac et al. Glucocortiocoid Treatment of MCMV Infected Newborn Mice Attenuates CNS Inflammation and Limits Deficits in Cerebellar Development. Cyclin D1 was detected in mouse cerebellar tissue using western blot
anti‐cyclin D3	31	Recombinant human cyclin D3 corresponding to residues 241‐260	Cell Signaling Cat# 2936 (AB_2070801)	1:1000	Barton et al. PD‐0332991, a CDK4/6 inhibitor, significantly prolongs survival in a genetically engineered mouse model of brainstem glioma. Cyclin D3 and pRb S780 were detected in glioblasotoma cells using western blot.
anti‐pRb phospho S780	110	Synthetic peptide corresponding to residues surrounding Ser780 of human Rb protein	Cell Signaling Cat# 8180 (AB_10950972)	1:1000
anti‐pRb phospho S795	110	Human Rb around the phosphorylation site of serine 795 (P‐S‐SP‐P‐L)	Cell Signaling Cat# ab47474 (AB_882295)	1:500	Ali et al. The development of a selective cyclin‐dependent kinase inhibitor which demonstrates anti‐tumor activity. pRb S795 was detected in MCF‐7 cells using western blot
anti‐pRb phospho S795	110	Human Rb around the phosphorylation site of serine 795 (P‐S‐SP‐P‐L)	Cell Signaling Cat# ab47474 (AB_882295)	1:500	Ali et al. The development of a selective cyclin‐dependent kinase inhibitor which demonstrates anti‐tumor activity. pRb S795 was detected in MCF‐7 cells using western blot
anti‐pRb phospho S807/811	110	Synthetic peptide corresponding to residues surrounding Ser807/811 of human Rb protein.	Cell Signaling Cat# 8516 (AB_11179075)	1:1000	Mo et al. MicroRNA‐610 suppresses the proliferation of human glioblastoma cells by repressing CCND2 and AKT3. pRb S807/811 and pRb total were detected in glioblasotoma cells using western blot.
anti‐pRb Total	110	Synthetic peptide corresponding to central residues of mouse Rb.	Cell Signaling Cat# 9313 (AB_1904119)	1:1000
anti‐cdk4	34	Purified recombinant cdk4 protein	Millipore Cat# MAB8879, Clone DCS‐35 (AB_2078833)	1:1000	Xu et al. Lin28 modulates cell growth and associates with a subset of cell cycle regulator mRNAs in mouse embryonic stem cells. Cdk4 was detected in mouse embryonic stem cells using western blot.
anti‐cdk2	33	Peptide corresponding to amino acids 287‐298 (C‐QDVTKPVPHLRL) of human cdk2	Millipore Cat# MAB8879, Clone DCS‐35 (AB_2078833)	1:1000	Rus et al. Sublytic Complement Attack Induces Cell Cycle in Oligodendrocytes. Cdk2 was detected in murine oligodendrocytes and oligodendrocyte progenitor cells using western blot.
anti‐cyclin E	52	Synthetic peptide corresponding to amino acids 378‐396 (GVLTPPHSSKKQSSEQETE) of rat Cyclin E	Millipore Cat# 07‐687 (AB_11213792)	1:1000	Ikenishi et al. Cell cycle regulation in mouse heart during embryonic and postnatal stages. Cyclin E was detected in murine embryonic cardiomyocytes using western blot.