Fig. 2.

Comparison of BzATP-induced Yo-Pro-1 uptake of humanized and murine P2X7R expressing peritoneal macrophages. a Pore formation capacity of peritoneal macrophages expressing the murine and the humanized P2X7R. BzATP concentration-response curves for the mouse and humanized P2X7R following different incubation times. The humanized receptor shows higher sensitivity toward the agonist compared to the murine counterpart as determined by increasing concentrations of BzATP. b Peritoneal macrophages expressing the murine or humanized P2X7R show differential modulation of Yo-Pro-1 uptake by TFP upon concurrent stimulation with BzATP. Two-way RM-ANOVA + Bonferroni post hoc test, *p < 0.05, **p < 0.005, n = 3 wells per group, with cells obtained of 2 animals per genotype. Data are expressed mean ± SEM